ABSTRACT
We investigated epidemiological characteristic of scrub typhus in Yongshan County,Yunnan Province,China. The serum samples were collected from the patients with fever for detecting the antibody against Orientia tsutsugamsushi (Ot) by colloidal gold immunoassay assay.Rat traps were used to capture rodents.The spleen tissues of the captured rodents were detected by nested-polymerase chain reaction for rickettsia groEL segment.The groEL segments were sequenced and analyzed the homology with the other known sequences.Thirty-four scrub typhus cases were found in Yongshan County,Yunnan Prov-ince from May 2015 to October 2015.Among them,21 cases were confirmed by laboratory tests and 13 cases were clinical di-agnosis diseases.Of these patients,32.35% of the cases occurred in June.The 32.35% were in the group of the 40-49 year-old,and 79.41% were farmers,94.12% exhibited eschar or skin ulcer(31.25% were observed in groin of these cases),and rash developed in 50%.In 39 spleen tissue samples of Rattus flavipectus,9 samples showed positive for groEL gene Ot,but gro-EL gene of Typhus group rickettsia and spotted fever group rickettsia were negative.Sequence analysis showed that YSP30 was closely related to some Saitama related strains of Ot,such as HSB1,FAR1 and UAP4,while the other 8 strains were closely related to some Karp related strains of Ot,such as UT213,UT221 and SH205.It was confirmed that the Yongshan County was the natural foci of scrub typhus by the serological and molecular biological detections.There are Karp and Saitama genotype related Ots in the natural foci.
ABSTRACT
Objective To investigate the pathophysiological role of serum hydrogen sulfide (H2 S) and its correlation with serum neuron-specific enolase (NSE) level in the process of febrile seizures (FS).Methods Sixty-five children with FS and acute upper respiratory infection were selected as FS group,51 children with acute upper respiratory infection associated with fever were taken as upper respiratory infection group and 43 healthy children in Child Health Section for physical examination were respectively chosen as the healthy control group.All children came from Department of Pediatrics of Zhujiang Hospital,Southern Medical University from Mar.15 to Nov.10,2012.The serum H2S and NSE levels of all groups were measured by Microplate Reader.Results The serum H2S in FS group was significantly lower than that in upper respiratory infection group and healthy control group (all P ＜ 0.01) ; the NSE level in FS group was significantly higher than that in upper respiratory infection group and healthy control group (all P ＜0.01) ;the levels of H2S and NSE had no significant differences between upper respiratory infection group and healthy control group (all P ＞ 0.05).The serum H2S level and NSE level in FS group were negatively correlated (r =-0.279,P =0.024) ; There were significant differences of serum NSE level between seizure frequency less than twice group and seizure frequency greater than or equal to twice group(t =-2.955,P =0.004).The seizure frequency was negatively correlated with serum H2S level (r =-0.269,P =0.03),and positively correlated with serum NSE level (r =0.322,P =0.009).The seizure durations (≥ 5 min) was negatively correlated with serum H2S level (r =-0.532,P =0.019).Conclusion The serum H2S level is expected to be an objective index for eva-luating the seizure brain injury in the early period of FS,and also potentially the important occurrence factors of brain injury.